Supplementary data.docx (395.26 kB)

A systematic investigation of key factors of nucleic acid precipitation towards optimized DNA/RNA isolation

dataset

posted on 2020-02-18, 09:30 authored by Yalin Li, Suxiang Chen, Nan Liu, Lixia Ma, Tao Wang, Rakesh N. Veedu, Tao Li, Fengqiu Zhang, Huiyue Zhou, Xiang Cheng, Xumiao Jing

Supplementary Figure 1 - Key influence factors and their corresponding conditions of nucleic acid precipitation;

Supplementary Table 1 - The average recovery rate and its corresponding standard deviation of each type of nucleic acid by adding alcohol and salt independently or both;

Supplementary Table 2 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each incubation condition;

Supplementary Table 3 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each centrifugation force;

Supplementary Table 4 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each centrifugation time;

Supplementary Table 5 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under each volume ratio of ethanol or isopropanol to nucleic acid solution;

Supplementary Table 6 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under different conditions of cations;

Supplementary Table 7 - The average recovery rate and its corresponding standard deviation of each type of nucleic acid upon addition of yeast tRNA as co-precipitator (carrier);

Supplementary Table 8 - The average recovery rate and its corresponding standard deviation and significance level of each type of nucleic acid under different conditions of carriers;

Supplementary Table 9 - The measuring results of carries in aqueous solutions by NanoDrop 2000.