10.25402/FSOA.9791603.v1
Ramme, Anja
Anja
Ramme
Koenig, Leopold
Leopold
Koenig
Hasenberg, Tobias
Tobias
Hasenberg
Schwenk, Christine
Christine
Schwenk
Magauer, Corinna
Corinna
Magauer
Faust, Daniel
Daniel
Faust
Lorenz, Alexandra
Alexandra
Lorenz
Krebs, Anna
Anna
Krebs
Drewell, Christopher
Christopher
Drewell
Schirrmann, Kerstin
Kerstin
Schirrmann
Grabovica, Alexandra
Alexandra
Grabovica
Lin, Grace
Grace
Lin
Pabinger, Stephan
Stephan
Pabinger
Neuhaus, Winfried
Winfried
Neuhaus
Bois, Frederic
Frederic
Bois
Lauster, Roland
Roland
Lauster
Marx, Uwe
Uwe
Marx
Dehne, Eva-Maria
Eva-Maria
Dehne
Supplementary material. Autologous iPSC-derived four-organ-chip – KeyGene counts
Future Science Group
2019
induced pluripotent stem cells
multi-organ-chip
four-organ-chip
differentiation
microphysiological system
Toxicology (incl. Clinical Toxicology)
Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
Cell Development, Proliferation and Death
Biochemistry and Cell Biology not elsewhere classified
2019-09-10 11:30:47
Dataset
https://future-science-group.figshare.com/articles/dataset/Supplementary_material_Autologous_iPSC-derived_four-organ-chip_KeyGene_counts/9791603
<div>Supplementary material. Autologous iPSC-derived four-organ-chip – KeyGene counts</div><div><br></div><div>Microphysiological systems play a pivotal role in progressing towards a global paradigm shift in drug development. Here, we designed a four-organ-chip interconnecting miniaturized human intestine, liver, brain and kidney equivalents. All four organ models were pre-differentiated from induced pluripotent stem cells from the same healthy donor and integrated into the microphysiological system. The co-culture of the four autologous tissue models in one common medium deprived of tissue specific growth factors was successful over 14-days. Although there were no added growth factors present in the co-culture medium, the intestine, liver and neuronal model maintained defined marker expression. Only the renal model was overgrown by coexisting cells and did not further differentiate. This model platform will pave the way for autologous co-culture cross-talk assays, disease induction and subsequent drug testing. </div><div><br></div>