10.25402/FSOA.9791603.v1 Ramme, Anja Anja Ramme Koenig, Leopold Leopold Koenig Hasenberg, Tobias Tobias Hasenberg Schwenk, Christine Christine Schwenk Magauer, Corinna Corinna Magauer Faust, Daniel Daniel Faust Lorenz, Alexandra Alexandra Lorenz Krebs, Anna Anna Krebs Drewell, Christopher Christopher Drewell Schirrmann, Kerstin Kerstin Schirrmann Grabovica, Alexandra Alexandra Grabovica Lin, Grace Grace Lin Pabinger, Stephan Stephan Pabinger Neuhaus, Winfried Winfried Neuhaus Bois, Frederic Frederic Bois Lauster, Roland Roland Lauster Marx, Uwe Uwe Marx Dehne, Eva-Maria Eva-Maria Dehne Supplementary material. Autologous iPSC-derived four-organ-chip – KeyGene counts Future Science Group 2019 induced pluripotent stem cells multi-organ-chip four-organ-chip differentiation microphysiological system Toxicology (incl. Clinical Toxicology) Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) Cell Development, Proliferation and Death Biochemistry and Cell Biology not elsewhere classified 2019-09-10 11:30:47 Dataset https://future-science-group.figshare.com/articles/dataset/Supplementary_material_Autologous_iPSC-derived_four-organ-chip_KeyGene_counts/9791603 <div>Supplementary material. Autologous iPSC-derived four-organ-chip – KeyGene counts</div><div><br></div><div>Microphysiological systems play a pivotal role in progressing towards a global paradigm shift in drug development. Here, we designed a four-organ-chip interconnecting miniaturized human intestine, liver, brain and kidney equivalents. All four organ models were pre-differentiated from induced pluripotent stem cells from the same healthy donor and integrated into the microphysiological system. The co-culture of the four autologous tissue models in one common medium deprived of tissue specific growth factors was successful over 14-days. Although there were no added growth factors present in the co-culture medium, the intestine, liver and neuronal model maintained defined marker expression. Only the renal model was overgrown by coexisting cells and did not further differentiate. This model platform will pave the way for autologous co-culture cross-talk assays, disease induction and subsequent drug testing. </div><div><br></div>