Supplementary Figure 2. Rapid Identification of Genome Edited Mesenchymal Stem Cell Colonies via Cas9
Wei Jiang
Wei Lian
Jieting Chen
Wenlei Li
Jieyong Huang
Baoyu Lai
Lingyun Li
Zhong Huang
Jianyong Xu
10.25402/BTN.7923737.v2
https://future-science-group.figshare.com/articles/journal_contribution/Supplementary_Figure_2_Target_inserting_the_IL-37_into_AASV1_site_/7923737
<div>
<div>
<table>
<tr>
<td>
<p><b>Supplementary Figure 2</b>. <b>Target inserting the IL-37 into AASV1
site. </b>(A) Donor and gRNA structures. (B) PCR characterization of genome
edited MSC colonies by amplifying the left arm. Arrow shows the predicted PCR
product size. (C) PCR characterization of genome edited MSC colonies by amplifying
the right arm. Arrow shows the predicted PCR product size. (D) mRNA level of
IL-37 among the colony 8 (C8) without donor insertion, colony 20 (C20) with
correct insertion, and the wild type of MSCs (NC). M: DNA ladder; arrows
showed the DNA size with 750bp, 1.0kb or 2kb.</p>
</td>
</tr>
</table>
</div>
<b></b><i></i><u></u><sub></sub><sup></sup><br></div>
2019-03-29 11:30:06
Mesenchymal stem cells
genome editing
Cas9
AAVS1
MSC
IL-10
IL-37
Direct PCR
Rapid identification
MSC colony
Genomics