Supplementary figure 3. Conducting polymer-based multilayer films for instructive biomaterial coatings
John G Hardy
Hetian Li
Jacqueline K Chow
Sydney A Geissler
Austin B McElroy
Lindsey Nguy
Derek S Hernandez
Christine E Schmidt
10.25402/FSOA.7873460.v2
https://future-science-group.figshare.com/articles/figure/Supplementary_figure_3_Conducting_polymer-based_multilayer_films_for_instructive_biomaterial_coatings/7873460
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<p><b>Supplementary Figure S3</b></p>
<p>HUMAN MESENCHYMAL
STEM CELL ADHESION STUDIES</p>
<p>HMSCs were supplied
by Lonza (Walkersville, MD). Samples were prepared as described above. After
sterilization, the samples were incubated for 30 minutes in 24 well plates
containing HMSC growth medium that was composed of: high glucose Dulbecco’s
Modified Eagle Medium (DMEM, 440 mL); fetal bovine serum (50 mL);
antibiotic-antimycotic (5 mL); non-essential amino acids (5 mL), and 2 ng
mL-1 basic fibroblast growth factor. Medium was aspirated and replaced prior
to HMSC seeding. Cell viability before starting the experiment was determined
by the Trypan Blue exclusion method, and the measured viability exceeded 95 %
in all cases. HMSCs were seeded at 5,000 cells cm-2, and incubated at 37 °C,
95 % humidity, and a CO2 content of 5 %. Samples were fixed and stained as
decribed above (n = 3).</p>
<p><b> </b>- HMSCs on multilayer
films after 3 days in culture. A) PEDOT-PSS-based multilayer films. B)
PEDOT-S-based multilayer films. DAPI-stained nuclei are blue and Alexa Fluor®
488-stained actin is green. Scale bars represent 150 µm.</p>
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2019-03-21 11:17:56
Biomaterials
cellular alignment
conducting polymers
Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
Cell Physiology
Synthetic Biology