Supplementary Figure 1: Strand specific detection of overlapping transcripts via purification involving denaturation of biotinylated cDNA
Faizan Uddin
Madhulika Srivastava
10.25402/BTN.12251249.v1
https://future-science-group.figshare.com/articles/figure/Supplementary_Figure_1_Strand_specific_detection_of_overlapping_transcripts_via_purification_involving_denaturation_of_biotinylated_cDNA/12251249
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<p><b>Supplementary
Figure 1.</b> Enrichment of biotinylated cDNA using
streptavidin magnetic beads. Antisense RNA was generated by in vitro
transcription of a plasmid clone having Eb region as the insert. 10ng of RNA
was reverse transcribed using biotinylated m1397 as primer. The resultant
cDNA was subjected to streptavidin magnetic beads based purification.
Abundance of cDNA before and after purification were estimated by qPCR
(m1397-m1398r). Relative levels of enriched biotinylated cDNA normalized to
total cDNA before enrichment in three cDNA samples are shown. Error bars
represent SEM (n=3). Primer locations as in Figure 4.</p>
<p><b>Supplementary
Table 1.</b> Sequences of the primers used in RT-PCR experiments</p>
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2020-05-06 09:23:15
Biotinylated primers
strand specific RT-PCR
self-priming
alkaline denaturation
streptavidin magnetic beads
detection of overlapping transcripts
Gene Expression (incl. Microarray and other genome-wide approaches)