10.25402/BTN.11949801.v1 Min Li Min Li Ling-Yan Jiang Ling-Yan Jiang Qin Liu Qin Liu Yuan-Hang Wu Yuan-Hang Wu Guo-Dao Liu Guo-Dao Liu Yin-Hua Chen Yin-Hua Chen Li-Juan Luo Li-Juan Luo A Method Combining TA Cloning and Fluorescence Screening for Rapid Acquisition of Transgenic Seeds Future Science Group 2020 Arabidopsis thaliana Stable expression vector OLE1 gene TA cloning Green fluorescent protein (GFP) Selection marker Plant Biology not elsewhere classified Gene Expression (incl. Microarray and other genome-wide approaches) 2020-03-06 14:17:31 Figure https://future-science-group.figshare.com/articles/figure/A_Method_Combining_TA_Cloning_and_Fluorescence_Screening_for_Rapid_Acquisition_of_Transgenic_Seeds/11949801 <div> <table> <tr> <td> <p><b>Fig S1 T</b><b>he picture of Gel electrophoresis during the constraction of pOGT vector</b><b></b></p> <p>(a) <a>Gel electrophoresis</a> of PCR amplified <i>Pro<sub>OLE1</sub>::OLE1</i> fragment (lane 1; amplified fragment: 2245 bp, indicated by arrow). (b) <i>Xho</i>Ⅰ and <i>Sal</i>Ⅰ digestion of the vector pA7-GFP (lane 2, 3; large fragment: 4591 bp; small fragment: 57 bp; both fragments were indicated by arrows). (c) Colony PCR from five random clones using primers pOLE1-F and pOLE1-R (lane 4-8; amplified fragment: 2245 bp; indicated by arrow). (d) Gel electrophoresis of PCR amplified <i>Pro<sub>OLE1</sub>::OLE1-GFP</i> fragment (lane 9, amplified fragment: 2989 bp; indicated by arrow). (e) <i>Hin</i>dⅢ digestion of the vector pCXSN (lane 10, 11; fragment: 10802 bp; indicated by arrow). (f) <i>Hin</i>dIII digestion of pCXSN-OLE1-GFP (lane 12, 13; large fragment: 10802 bp; small fragment: 2957 bp; both fragments were indicated by arrows ). (g) <i>Xcm</i>I digestion of the vector pOGT with mutated promoter and the pCXSN-OLE1-GFP with original promoter. The asterisks indicated the resulting fragments after <i>Xcm</i>I digestion. The last lane was the undigested pOGT vector. </p> <p><b>Fig S2 PCR amplification of <i>GFP</i>, <i>HygR</i>, <i>ACT</i>2 fragment from T<sub>1</sub> transgenic plants expressing pOGT. </b><b></b></p> <p>Lane 1-20 shows the amplification from 20 independent T<sub>1</sub> plants. Lane 21 shows the amplification from <a>Arabidopsis wild type plants</a> (Col-0). Lane 22 shows the amplification with no template. Lane 23 shows the amplification from pOGT plasmid. <i>GFP</i><a> fragment</a>: 272 bp, <i>HygR</i> fragment: 444 bp, <i>ACT2</i> fragment: 188 bp.</p> </td> </tr> </table> </div>