A systematic investigation of key factors of nucleic acid precipitation towards optimized DNA/RNA isolation
Yalin Li
Suxiang Chen
Nan Liu
Lixia Ma
Tao Wang
Rakesh N. Veedu
Tao Li
Fengqiu Zhang
Huiyue Zhou
Xiang Cheng
Xumiao Jing
10.25402/BTN.11864649.v1
https://future-science-group.figshare.com/articles/dataset/A_systematic_investigation_of_key_factors_of_nucleic_acid_precipitation_towards_optimized_DNA_RNA_isolation/11864649
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<p>Supplementary Figure
1 - Key influence factors and their corresponding conditions of nucleic acid
precipitation;</p>
<p>Supplementary Table 1
- The average recovery rate and its corresponding standard deviation of each
type of nucleic acid by adding alcohol and salt independently or both;</p>
<p>Supplementary Table 2
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under each incubation
condition;</p>
<p>Supplementary Table 3
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under each centrifugation
force;</p>
<p>Supplementary Table 4
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under each centrifugation
time;</p>
<p>Supplementary Table 5
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under each volume ratio of
ethanol or isopropanol to nucleic acid solution;</p>
<p>Supplementary Table 6
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under different conditions of
cations;</p>
<p>Supplementary Table 7
- The average recovery rate and its corresponding standard deviation of each
type of nucleic acid upon addition of yeast tRNA as co-precipitator
(carrier);</p><div>
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<p>Supplementary Table 8
- The average recovery rate and its corresponding standard deviation and
significance level of each type of nucleic acid under different conditions of
carriers;</p>
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Supplementary Table 9 - The measuring results of
carries in aqueous solutions by NanoDrop 2000.<br></p>
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2020-02-18 09:30:48
Nucleic acid precipitation
Ethanol
Isopropanol
Linear polyacrylamide (LPA)
Genetics not elsewhere classified