10.25402/BTN.11353907.v1
Tomo Kondo
Tomo
Kondo
Shigehiko Yumura
Shigehiko
Yumura
Supplementary Figure S4. Simultaneous multiple protein expression from a single vector for Dictyostelium
Future Science Group
2019
E. coli
molecular cloning
screening
GFP
Dictyostelium
Magnetospirillum
Microbial Genetics
Genomics
2019-12-11 16:24:04
Figure
https://future-science-group.figshare.com/articles/figure/Supplementary_Figure_S4_Simultaneous_multiple_protein_expression_from_a_single_vector_for_Dictyostelium/11353907
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<b>Supplementary Figure S4. Simultaneous multiple
protein expression from a single vector for Dictyostelium.</b> (A) Scheme of tandem GOI alignment using
seamless cloning (In-fusion cloning). After linearization of the vector
containing GOI1 with ApaI (whose recognition site is not present in 99.5% of
the Dictyostelium genes), a PCR product containing GOI2 with a promoter
(orange) and terminator (cyan, shaded) was inserted. (B) A γ-tubulin–GFP
expressing cell. G418 was used for selection. (C) A γ-tubulin–GFP and
mRuby3–histone H1 expressing cell. Only G418 was used for selection. The right
side of each image superimposed to the phase-contrast image. Bars, 10 µm.