10.25402/BTN.11353907.v1 Tomo Kondo Tomo Kondo Shigehiko Yumura Shigehiko Yumura Supplementary Figure S4. Simultaneous multiple protein expression from a single vector for Dictyostelium Future Science Group 2019 E. coli molecular cloning screening GFP Dictyostelium Magnetospirillum Microbial Genetics Genomics 2019-12-11 16:24:04 Figure https://future-science-group.figshare.com/articles/figure/Supplementary_Figure_S4_Simultaneous_multiple_protein_expression_from_a_single_vector_for_Dictyostelium/11353907 <div> </div> <b>Supplementary Figure S4. Simultaneous multiple protein expression from a single vector for Dictyostelium.</b> (A) Scheme of tandem GOI alignment using seamless cloning (In-fusion cloning). After linearization of the vector containing GOI1 with ApaI (whose recognition site is not present in 99.5% of the Dictyostelium genes), a PCR product containing GOI2 with a promoter (orange) and terminator (cyan, shaded) was inserted. (B) A γ-tubulin–GFP expressing cell. G418 was used for selection. (C) A γ-tubulin–GFP and mRuby3–histone H1 expressing cell. Only G418 was used for selection. The right side of each image superimposed to the phase-contrast image. Bars, 10 µm.