10.25402/BTN.11282930.v1
Pei-Zhong Tang
Pei-Zhong
Tang
Bo Ding
Bo
Ding
Lansha Peng
Lansha
Peng
Vadim Mozhayskiy
Vadim
Mozhayskiy
Jason Potter
Jason
Potter
Jonathan D. Chesnut
Jonathan D.
Chesnut
Supplementary material for TEG-seq: an ion torrent-adapted NGS workflow for in cellulo mapping of CRISPR specificity
Future Science Group
2019
CRISPR/cas9
gene editing
GUIDE-seq
off-target
TEG-seq
Gene Expression (incl. Microarray and other genome-wide approaches)
Genetics not elsewhere classified
2019-11-27 14:06:09
Dataset
https://future-science-group.figshare.com/articles/dataset/Supplementary_material_for_TEG-seq_an_ion_torrent-adapted_NGS_workflow_for_in_cellulo_mapping_of_CRISPR_specificity/11282930
<div>
<table>
<tr>
<td>
<p>Supplementary Figure
1: Ion-GUIDE-seq workflow</p>
<p> </p>
<p>Supplementary Figure
2: PCR amplification using different primer pairs</p>
<p> </p>
<p>Supplementary Table
1: gRNA sequence and In silico design
score from MIT design tool </p>
<p> </p>
<p>Supplementary Table
2: TEG-seq and targeted amplicon-seq data
</p>
<p> </p>
<p>Supplementary Table
3: Data from PGM and Proton for HEK4</p>
<p> </p>
<p>Supplementary Table
4: 22 gRNAs designed to target eight genetic disorder relevant SNPs</p>
<p> </p><p>Supplementary Table
5: Oligo sequences of different primers and adaptors</p><p></p>
<p> </p>
</td>
</tr>
</table>
</div>
Protocol for TEG-seq: An Ion Torrent-adapted NGS
workflow for <i>in cellulo</i> mapping of CRISPR specificity