10.25402/BTN.11282930.v1 Pei-Zhong Tang Pei-Zhong Tang Bo Ding Bo Ding Lansha Peng Lansha Peng Vadim Mozhayskiy Vadim Mozhayskiy Jason Potter Jason Potter Jonathan D. Chesnut Jonathan D. Chesnut Supplementary material for TEG-seq: an ion torrent-adapted NGS workflow for in cellulo mapping of CRISPR specificity Future Science Group 2019 CRISPR/cas9 gene editing GUIDE-seq off-target TEG-seq Gene Expression (incl. Microarray and other genome-wide approaches) Genetics not elsewhere classified 2019-11-27 14:06:09 Dataset https://future-science-group.figshare.com/articles/dataset/Supplementary_material_for_TEG-seq_an_ion_torrent-adapted_NGS_workflow_for_in_cellulo_mapping_of_CRISPR_specificity/11282930 <div> <table> <tr> <td> <p>Supplementary Figure 1: Ion-GUIDE-seq workflow</p> <p> </p> <p>Supplementary Figure 2: PCR amplification using different primer pairs</p> <p> </p> <p>Supplementary Table 1: gRNA sequence and In silico design score from MIT design tool </p> <p> </p> <p>Supplementary Table 2: TEG-seq and targeted amplicon-seq data </p> <p> </p> <p>Supplementary Table 3: Data from PGM and Proton for HEK4</p> <p> </p> <p>Supplementary Table 4: 22 gRNAs designed to target eight genetic disorder relevant SNPs</p> <p> </p><p>Supplementary Table 5: Oligo sequences of different primers and adaptors</p><p></p> <p> </p> </td> </tr> </table> </div> Protocol for TEG-seq: An Ion Torrent-adapted NGS workflow for <i>in cellulo</i> mapping of CRISPR specificity